counting cardiocytes

Frank O. Fackelmayer Frank.Fackelmayer at uni-konstanz.de
Thu Feb 24 15:52:04 EST 2000


christian wrote:

> In article <spam-0402001429280001 at macmac-2.ucsf.edu>,
> spam at 127.0.0.1 (Bernard Murray, PhD) wrote:
> > In article
> > <019bf6ce.36646537 at usw-ex0109-069.remarq.com>,
> > christian
> > <christian.troidlNOchSPAM at kerckhoff.mpg.de.invalid>
> > wrote:
> > > I want to count beta-Gal transfected cardiocytes on a
> > > petridish!
> > Rather than a gridded dish you can use a microscope
> > eyepiece
> > insert (graticule) that has a grid upon it.  The other
> > similar
> > way is with a graticule that has a random pattern of
> > dots
> > on it and you then count the proportion of dots
> > landing on
> > stained cells to give yourself a transfection
> > efficiency.
> > I've used the former method for a variety of b-gal
> > transfected
> > cell lines.
> >    Bernard
> > --
> > Bernard P. Murray, PhD
> > bpmurray at cgl . ucsf . edu
> > Department of Cellular & Molecular Pharmacology, UCSF
>
> dear bernard
>
> thank you very much for your answer to my request.
>
> as you maby realized I'm german and I have problems with the
> word "graticule". could you specifie what you mean by that.

graticule heißt "Raster", sagt zumindest das Wörterbuch unter
http://www.tu-chemnitz.de/urz/netz/forms/dict.html

Ich kannte das Wort auch nicht....

Viel Glück,
Frank





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