need help

[Gautam Sondarva]

Hello!!

I am looking for assistance in making spleenocytes from mice.
How do I go about doing it?

I shall be happy if someone can give me directions as to where
I can find a method OR if someone can be kind enough to send
me a step-by-step protocol for the same.

I am aiming at immortalizing these spleenocytes, hence would be
happy if someone can give me additional information for the same.
OR atleast a method which will allow me to maintain these cells for
a short time in tissue culture.

Thanking you all in advance
Gautam



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