Skeletal muscle cell media
expell at hotmail.com
Thu Jan 13 23:05:34 EST 2000
By alive I mean healthy looking and still able to contract upon stimulation.
I'm using dissected single muscle fibres from 8 week old mice and want to keep
them alive that's all. No cell lines or cultures. Straight out of the mouse.
Courtland Yockey wrote:
> Could you be more specific about your problem?
> What exactly do you mean by "alive"? Attached? Healthy-looking?
> What cell type are you talking about: Primary fiber explants? Primary
> culture myoblasts that have been induced to differentiate? Cell lines that
> have been induced to differentiate?
> courtland.yockey at mindspring.com
> In article <387DB461.D69D5C18 at hotmail.com>, Viet <expell at hotmail.com>
> I need help in finding media in which to keep terminally differentiated
> skeletal muscle cells alive for over 3 days. As of yet I have not been
> able to find anything in the literature. There is plenty on primary
> cell cultures but nothing on what I am looking for. I refuse to believe
> that no one has tried to keep terminally differentiated skeletal muscle
> cells alive for any period of time. Any help would be most appreciated.
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