Skeletal muscle cell media

Viet expell at hotmail.com
Thu Jan 13 23:05:34 EST 2000


By alive I mean healthy looking and still able to contract upon stimulation.

I'm using dissected single muscle fibres from 8 week old mice and want to keep
them alive that's all.  No cell lines or cultures.  Straight out of the mouse.


Courtland Yockey wrote:

> Viet:
>
> Could you be more specific about your problem?
>
> What exactly do you mean by "alive"?  Attached?  Healthy-looking?
>
> What cell type are you talking about:  Primary fiber explants?  Primary
> culture myoblasts that have been induced to differentiate?  Cell lines that
> have been induced to differentiate?
>
> courtland.yockey at mindspring.com
>
> =============
> In article <387DB461.D69D5C18 at hotmail.com>, Viet <expell at hotmail.com>
> wrote:
>
> I need help in finding media in which to keep terminally differentiated
> skeletal muscle cells alive for over 3 days.  As of yet I have not been
> able to find anything in the literature.  There is plenty on primary
> cell cultures but nothing on what I am looking for.  I refuse to believe
>
> that no one has tried to keep terminally differentiated skeletal muscle
> cells alive for any period of time.  Any help would be most appreciated.
>
> Thanks

--
Viet Pham

Department of Physiology
The University of Sydney
NSW 2006 AUSTRALIA

E-mail:  viet at physiol.usyd.edu.au
Phone:  +61 2 9351 4526
Mobile:  +61 412 321 279
Fax:  +61 2 9351 2058






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