PCR

Beat at hgmp.mrc.ac.uk Beat at hgmp.mrc.ac.uk
Sun Jan 23 04:27:20 EST 2000


Hi!
I used to do that by extracting the bands and perform a dot blot.
Subcloning and sequencing would be the other solution...

Beat



On Sun, 23 Jan 2000 15:47:26 +0800, "Chong Wai Yin( Zhang Weixian)"
<wychong at singnet.com.sg> wrote:

>Hi, is there anyway to know if the extra bands after PCR is due to
>unspecific binding or due to fragmented DNA-of-interest. (eg. gene of
>interest is fragmented and after PCR, the fragment is amplified.). Thanks.
>
>--
>Chong Wai Yin, Kelvin
>Zhang Weixian
>-- For Nature, heartless, witless Nature
>   Will neither know nor care.
>   And from my pillow, looking forth by light
>   Of moon or favouring stars, I could behold
>   The antechapel where the statue stood
>   Of Newton with his prism and silent face,
>   The marble index of a mind for ever
>   Voyaging through strange seas of Thought, alone. --
>
>





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