NEURON CULTURES ON COVERSLIPS??

[Martin Weber]

Hi folks!

Gotta grow embryonic neurons on coverslips for fluorescence microscopy
experiments with fura-2 or fluo3. 

Problems:

1) Neurons don't grow very well on (poly-l-ornithine coated) GLASS
coverslips.

2) Some (all???) PLASTIC coverslips show autofluorescence which can
confuse the fluorescence measurements.

Would be very glad about any suggestions, hints etc.

Regards, Martin