Gene Targeting by Homologous Recombination in Drosophila

Rcjohnsen rcjohnsen at aol.com
Sun Jul 2 13:25:41 EST 2000


Gene Targeting by Homologous Recombination in Drosophila 

Yikang S. Rong and Kent G. Golic * 
Drosophila offers many advantages as an experimental organism. However, in
comparison with yeast and mouse, two other widely used eukaryotic model
systems, Drosophila suffers from an inability to perform homologous
recombination between introduced DNA and the corresponding chromosomal loci.
The ability to specifically modify the genomes of yeast and mouse provides a
quick and easy way to generate or rescue mutations in genes for which a DNA
clone or sequence is available. A method is described that enables analogous
manipulations of the Drosophila genome. This technique may also be applicable
to other organisms for which gene-targeting procedures do not yet exist. 
Department of Biology, University of Utah, Salt Lake City, UT 84112, USA. 
*   To whom correspondence should be addressed. E-mail:
golic at bioscience.utah.edu 






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