Plasmid question

Chris LaRosa clarosa at biocomp.unl.edu
Thu Jul 27 13:03:49 EST 2000



Mark Garry Petten wrote:
> 
> I am running a sample of plasmid I have ligated with my cDNA insert
> through a 1% TBE gel. I am also running with it a sample of linearized
> plasmid without the insert. My question is will supercoiling cause the
> ligated plamid to migrate less through the gel or more?
> 
> ***

supercoiling without EtBR will cause plasmids to migrate faster, ahead
of linearized plasmids.   Addition of EtBr changes the conformation, and
I think results in plasmid DNA moving more like nonsupercoiled circular
dNA






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