Plasmid question

Martin Offterdinger martin.offterdinger at akh-wien.ac.at
Fri Jul 28 06:02:02 EST 2000


Chris LaRosa <clarosa at biocomp.unl.edu> schrieb in im Newsbeitrag:
39807985.9C81AF42 at biocomp.unl.edu...
>
>
> Mark Garry Petten wrote:
> >
> > I am running a sample of plasmid I have ligated with my cDNA insert
> > through a 1% TBE gel. I am also running with it a sample of linearized
> > plasmid without the insert. My question is will supercoiling cause the
> > ligated plamid to migrate less through the gel or more?
> >
> > ***
>
> supercoiling without EtBR will cause plasmids to migrate faster, ahead
> of linearized plasmids.   Addition of EtBr changes the conformation, and
> I think results in plasmid DNA moving more like nonsupercoiled circular
> dNA
Chris is right, but ligation products will NEVER be in supercoiled form!!!
Martin







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