Plasmid question

Chris LaRosa clarosa at biocomp.unl.edu
Fri Jul 28 09:08:47 EST 2000



Martin Offterdinger wrote:
> 
> Chris LaRosa <clarosa at biocomp.unl.edu> schrieb in im Newsbeitrag:
> 39807985.9C81AF42 at biocomp.unl.edu...
> >
> >
> > Mark Garry Petten wrote:
> > >
> > > I am running a sample of plasmid I have ligated with my cDNA insert
> > > through a 1% TBE gel. I am also running with it a sample of linearized
> > > plasmid without the insert. My question is will supercoiling cause the
> > > ligated plamid to migrate less through the gel or more?
> > >
> > > ***
> >
> > supercoiling without EtBR will cause plasmids to migrate faster, ahead
> > of linearized plasmids.   Addition of EtBr changes the conformation, and
> > I think results in plasmid DNA moving more like nonsupercoiled circular
> > dNA



> Chris is right, but ligation products will NEVER be in supercoiled form!!!
> Martin

True ...






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