Plasmid question

Dr. Mary Sarcina m.sarcina at ucl.ac.uk
Fri Jul 28 10:33:44 EST 2000


Hi Mark,

Your "clone" will supercoil as well, of course it might migrate differently
from the wild type it depends on the size of your insert. the best way it to
digest it and then run it on the gel.

Best of luck
Mary


Mark Garry Petten wrote:

> I am running a sample of plasmid I have ligated with my cDNA insert
> through a 1% TBE gel. I am also running with it a sample of linearized
> plasmid without the insert. My question is will supercoiling cause the
> ligated plamid to migrate less through the gel or more?
>
> *************************************************************************
> *Mark G. Petten                      * Research is the process of going *
> *Dept. of Biology                    * up alley's to see if they're     *
> *Honours                             * blind.                           *
> *Memorial University of Newfoundland * - Bates's Law of Research        *
> *************************************************************************






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