Genetic Program of Hematopoietic Stem Cells

Rcjohnsen rcjohnsen at
Tue Jun 6 17:33:24 EST 2000

The Genetic Program of Hematopoietic Stem Cells 

Robert L. Phillips, 1 Robin E. Ernst, 1 Brian Brunk, 2 Natalia Ivanova, 1 Mark
A. Mahan, 1 Julia K. Deanehan, 1 Kateri A. Moore, 1 G. Christian Overton, 2
Ihor R. Lemischka 1* 
Blood cell production originates from a rare population of multipotent,
self-renewing stem cells. A genome-wide gene expression analysis was performed
in order to define regulatory pathways in stem cells as well as their global
genetic program. Subtracted complementary DNA libraries from highly purified
murine fetal liver stem cells were analyzed with bioinformatic and array
hybridization strategies. A large percentage of the several thousand gene
products that have been characterized correspond to previously undescribed
molecules with properties suggestive of regulatory functions. The complete
data, available in a biological process-oriented database, represent the
molecular phenotype of the hematopoietic stem cell. 
1 Department of Molecular Biology, Princeton University, Princeton, NJ
08544, USA. 
2 Computational Biology and Informatics Laboratory, University of Pennsylvania,
Philadelphia, PA 19104, USA. 
*   To whom correspondence should be addressed. E-mail:
ilemischka at 
Science 288 june 1635 Phillips et. al.

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