Sci 288:(5474)2212

Rcjohnsen rcjohnsen at
Fri Jun 23 23:50:24 EST 2000

Molecular Biology   

Bacterial Mode of Replication with Eukaryotic-Like Machinery in a
Hyperthermophilic Archaeon 

Hannu Myllykallio, 1 Philippe Lopez, 2 Purificación López-García, 1* Roland
Heilig, 3 William Saurin, 3 Yvan Zivanovic, 1 Hervé Philippe, 2 Patrick
Forterre 1 
Despite a rapid increase in the amount of available archaeal sequence
information, little is known about the duplication of genetic material in the
third domain of life. We identified a single origin of bidirectional
replication in Pyrococcus abyssi by means of in silico analyses of cumulative
oligomer skew and the identification of an early replicating chromosomal
segment. The replication origin in three Pyrococcus species was found to be
highly conserved, and several eukaryotic-like DNA replication genes were
clustered around it. As in Bacteria, the chromosomal region containing the
replication terminus was a hot spot of genome shuffling. Thus, although
bacterial and archaeal replication proteins differ profoundly, they are used to
replicate chromosomes in a similar manner in both prokaryotic domains.  
1 Institut de Génétique et Microbiologie, 
2 Laboratoire de Biologie Cellulaire, Université de Paris-Sud, 91405 Orsay
Cedex, France. 
3 Génoscope, Centre National de Séquençage, Evry, France. 
*   Present address: Department of Microbiology, Universidad Miguel Hernández,
03550 San Juan de Alicante, Spain.  

   To whom correspondence should be addressed. E-mail:
forterre at 

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