Fibroblasts in fusion plates

Joe Chandler jchandler at mainebiotechnology.com
Tue Jun 27 16:30:03 EST 2000


Hi all,
We have a few fusions, particularly in rat spleen cells x mouse myeloma,
where there is an preponderance of fibroblasts growing in  the fusion plate.
There does not appear to be a relationship between the immungen and the
occurance of the fibroblasts (but I haven't thoroughly analyzed this
possibility).  Very often the fibroblasts starve the burgeoning hybridomas
and we loose them.  I vaguely remember a kind of plate or some procedure
that thwarted fibroblast growth.  My recollection was that you used these
plates or followed this procedure when you were trying to grow primary
endothelial cells as fibroblasts were a common nuisance.  Any ideas will be
greatly appreciated.
Joe







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