IP3/Ca2+ signaling

Leon l.j.brokken at amc.uva.nl
Wed Mar 1 11:24:01 EST 2000


Hi collegues,

What is the easiest/fastest way to determine whether the IP3/Ca2+
pathway is activated when I stimulate my mouse pituitary cell line?
Initially I thought of Ca-imaging using CLSM, but we do not have a
UV-laser, and fluorscent Ca-probes with excitation in the visible light
range tend to load difficult, I am told. Does anyone have experience
with these probes?

Best regards,

Leon Brokken
Academic Medical Center
Amsterdam
The Netherlands




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