HELP!!!! how to purify protein require for NAD
ssultang at dreamx.net
Fri Mar 3 19:11:20 EST 2000
I am purifying some aldehyde dehydrogenases requiring NAD with
But,I could not find the activity after 5'AMP-sepharose 4B affinity
In column work,
- starting and washing buffer : 10mM sodium phosphate buffer
- eluting bnuffer : containing 0.5, 3, 7,10mM NAD
- and at last eluting with 100mM sodium phosphate buffer.
Where is the enzyme?
How can I detach the guys from 5'AMP-sepharose 4B?
Please send me your idea.
With best regards.
Thank you in advance for your reply......
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