hematocymeters

B. necrotic at mailcity.com
Thu Mar 9 14:33:58 EST 2000



Avg. # of cells per chamber * dulution factor * 1E4 = cells/mL

ie.

300uL PBS
500uL Trypan
200uL Cell Suspension

this yields an average of 43 cells per chamber

so  43 * 5 * 1E4 = 2.15E6 cells/mL


this is how I've always done it.

B.


In article <zUOx4.842$5O2.9586 at newsr1.maine.rr.com>, "Joe Chandler"
<jchandler at mainebiotechnology.com> wrote:

> Isn't it:
> 
> (Cell count/number of chambers counted) * dilution * 1000 = cells/mL
> 
> 
> <katsman64 at my-deja.com> wrote in message news:8a86of$d82$1 at nnrp1.deja.com...
> > I'd like to ask about the Malasser
> > hematocymeter...
> > Which is the right method to calculate the total
> > number of cells?
> > Is it No cells * dilution * 10oooo = total number
> > of cells....
> >
> >
> > Sent via Deja.com http://www.deja.com/
> > Before you buy.




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