help!how to detect cytochrome C releasing from motocondria!
Paul S. Brookes.
brookes at uab.edu
Mon Mar 13 14:05:17 EST 2000
Lyse (gently) the cells and centrifuge. Then western blot for cyt-c in the
mitochondrial pellet and the cytosol/supernatant. Pharmingen's mouse
monoclonal Ab works well in our hands. See FASEB J. vol 13, pp2031-2036
(1999) for a recent example of this.
Of course with any such method you have to make sure any cyt-c you see in
the supernatant is actually released and not due to non-pelleted
mitochondria. Enzyme assays like citrate synthase are simple enough to
Dr. Paul S. Brookes. (brookes at uab.edu)
UAB Department of Pathology, G004 Volker Hall
1670 University Blvd., Birmingham AL 35294 USA
Tel (001) 205 934 1915 Fax (001) 205 934 1775
The quality of e-mails can go down as well as up
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