Fetal calf serum
Bernard Murray, PhD
spam at 127.0.0.1
Tue Mar 14 16:30:32 EST 2000
In article <38CEDF43.4EC2 at le.ac.uk>, "A.F. Simpson" <AFS7 at le.ac.uk> wrote:
> Northern Boy wrote:
> > Almost all cells are a lot happier in foetal calf serum, and proliferate
> > much more.
> I was once told by someone that HEK293 cells gave far higher
> transfection efficiencies if grown in newborn serum rather than foetal
> serum. Of course, that may have just been the HEK293 cells in their
NIH3T3 fibroblasts should definitely not be grown in FCS or they
will start to act very weird (transformation?). Growth in
newborn calf serum will prevent this.
Otherwise I agree with "Almost all cells...".
Bernard P. Murray, PhD
bpmurray at cgl . ucsf . edu
Department of Cellular & Molecular Pharmacology, UCSF
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