Fetal calf serum

Bernard Murray, PhD spam at 127.0.0.1
Tue Mar 14 16:30:32 EST 2000


In article <38CEDF43.4EC2 at le.ac.uk>, "A.F. Simpson" <AFS7 at le.ac.uk> wrote:

> Northern Boy wrote:
> > 
> > Almost all cells are a lot happier in foetal calf serum, and proliferate
> > much more.
> 
> I was once told by someone that HEK293 cells gave far higher
> transfection efficiencies if grown in newborn serum rather than foetal
> serum.  Of course, that may have just been the HEK293 cells in their
> lab.

NIH3T3 fibroblasts should definitely not be grown in FCS or they
will start to act very weird (transformation?).  Growth in
newborn calf serum will prevent this.
   Otherwise I agree with "Almost all cells...".

   Bernard

-- 
Bernard P. Murray, PhD
bpmurray at cgl . ucsf . edu
Department of Cellular & Molecular Pharmacology, UCSF




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