3T3 mouse fibroblasts
jaymone at paonline.com
Sat Mar 18 10:39:27 EST 2000
All I can give is my personal experience with both glycerol and DMSO. I
have found that for most of the cells I have worked with in the past, I have
gotten better viability with growth media supplemented with 10% glycerol
than media with 10% DMSO.
FYI, I was working in a lab which used human and monkey lymphocytes. In
this lab we froze the lymphocytes in fetal bovine serum with 10% glycerol,
and got excellent viability.
More information about the Cellbiol