3T3 mouse fibroblasts

Jay Mone jaymone at paonline.com
Sat Mar 18 10:39:27 EST 2000


Guenter,
All I can give is my personal experience with both glycerol and DMSO.  I
have found that for most of the cells I have worked with in the past, I have
gotten better viability with growth media supplemented with 10% glycerol
than media with 10% DMSO.

FYI, I was working in a lab which used human and monkey lymphocytes.  In
this lab we froze the lymphocytes in fetal bovine serum with 10% glycerol,
and got excellent viability.

Jay Mone'
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