replaceing Triton

Chris LaRosa clarosa at biocomp.unl.edu
Mon May 8 15:49:50 EST 2000



Tomáš Belza wrote:
> 
> Hallo,
> my question is : how is it possible to remove triton from the sample
> with diluted protein , proteins should be kept active and intact and
> should be put onto cells.
> i tried to use gel filtration (sephadex 50), but it doesn work. any
> advice could help !
> same problem with azide ....
> thanks
> tomas
> 

Possibly dialysis or membrane filtration , concentration and redilution.
Complete removal will be very difficult without repeated rounds of any
detergent or salt removal routine.




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