replaceing Triton

Chris LaRosa clarosa at
Mon May 8 15:49:50 EST 2000

Tomáš Belza wrote:
> Hallo,
> my question is : how is it possible to remove triton from the sample
> with diluted protein , proteins should be kept active and intact and
> should be put onto cells.
> i tried to use gel filtration (sephadex 50), but it doesn work. any
> advice could help !
> same problem with azide ....
> thanks
> tomas

Possibly dialysis or membrane filtration , concentration and redilution.
Complete removal will be very difficult without repeated rounds of any
detergent or salt removal routine.

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