the need for dowex and alumina in cAMP assays
hines at pharm.med.upenn.edu
Sat May 20 14:39:44 EST 2000
In article <hines-1805002033030001 at rm162e-3.vetschool.upenn.edu>,
hines at pharm.med.upenn.edu (John Hines) wrote:
> What the chapter doesn't really explain is the reason for the subsequent
> alumina column chromatography. If the Dowex step eliminates the
> overwhelming majority of the 3H-ATP from your 3H-cAMP, then why not elute
> the 3H-cAMP from the Dowex directly into scintillation cocktail? Why do
> you have the alumina column step?
> Obviously, if so many labs bother with the alumina step, it must have a
> benefit. I'm just curious to know what that benefit is.
No one knows?
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