the need for dowex and alumina in cAMP assays

[John Hines]

In article <hines-1805002033030001 at rm162e-3.vetschool.upenn.edu>,
hines at pharm.med.upenn.edu (John Hines) wrote:

 
> What the chapter doesn't really explain is the reason for the subsequent
> alumina column chromatography.  If the Dowex step eliminates the
> overwhelming majority of the 3H-ATP from your 3H-cAMP, then why not elute
> the 3H-cAMP from the Dowex directly into scintillation cocktail? Why do
> you have the alumina column step?
> 
> 
> Obviously, if so many labs bother with the alumina step, it must have a
> benefit.  I'm just curious to know what that benefit is.
> 


No one knows?

John