Charcoal stripped FBS vs Heat inactivated FBS
"srohrer(removethis)" at immv.unizh.ch
Wed Nov 29 09:42:24 EST 2000
Martin Offterdinger wrote:
> Yes, there is a difference indeed, the two methods have nothing at all in
> -Heat treatment inactivates complement and should always be done with FCS!
> -Charcoal treatment removes hydrophobic, low MW compounds (i.e. steroid
> hormones, etc...)
> It is troublesome to prepare charcoal stripped serum (takes about 1 week of
> stírring with charcoal and even more troublesome removing charcoal
> afterwards by filtration!!)
We used a protocol for dextran-coated charcoal treatment of FBS once a long
time ago (van der Eijnden-van Raij et al, Mech. Dev. 1991) that takes a day or
so. maybe that helps, maybe it removes too much stuff (it did in my case)
It's been a long time, so I take no responsibility for this.
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