Proteinase K

Pamela Norton pamela.norton at mail.tju.edu
Thu Oct 5 12:09:49 EST 2000


In article <8M1D5.112542$i5.4858406 at news1.rdc2.on.home.com>, Jen
Robertson <jrobertson6 at home.com> wrote:

> I just make it up 10mg/ml in distilled water. Haven't had a problem with it
> at all. Stored at -20 and +4.
> 
> 
> Heike Will <will at uni-muenster.de> wrote in message
> news:39DC5450.65A7693A at uni-muenster.de...
> > I have problems to store Proteinase K. After a short time, this enzyme
> > is inactive. i have stored it at +4°C, at -20°C and at -80°C.
> > In different Tris/HCL-buffers with and without NaCl.
> >

We store at -20 in Tris. However, it should be aliquoted, as repeated
freeze/thawing leads to autodigestion and loss of activity.

Pam






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