Proteinase K

Heike Will will at uni-muenster.de
Fri Oct 6 03:58:44 EST 2000


what is the Tris - and the Proteinase K concentration?

Pamela Norton wrote:

> In article <8M1D5.112542$i5.4858406 at news1.rdc2.on.home.com>, Jen
> Robertson <jrobertson6 at home.com> wrote:
>
> > I just make it up 10mg/ml in distilled water. Haven't had a problem with it
> > at all. Stored at -20 and +4.
> >
> >
> > Heike Will <will at uni-muenster.de> wrote in message
> > news:39DC5450.65A7693A at uni-muenster.de...
> > > I have problems to store Proteinase K. After a short time, this enzyme
> > > is inactive. i have stored it at +4°C, at -20°C and at -80°C.
> > > In different Tris/HCL-buffers with and without NaCl.
> > >
>
> We store at -20 in Tris. However, it should be aliquoted, as repeated
> freeze/thawing leads to autodigestion and loss of activity.
>
> Pam
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