Proteinase K

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Fri Oct 6 20:23:50 EST 2000


In article <061020001404280743%pamela.norton at mail.tju.edu>,
  Pamela Norton <pamela.norton at mail.tju.edu> wrote:

[...]

>
> Okay, now you've got me checking. The enzyme is dissolved at 10 mg/ml.
> I have one protocol that says dissolve in water, another protocol
> suggests 50 mM Tris, 5 mM EDTA, pH 8. I had 10 mM Tris pH 8 in mind
> when I originally posted; I'm guessing that all will work. Good luck,
>

I've been making mine at 10 mg/ml in water, aliquoted, and stored at -
80C.

Nick

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Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu


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