Protein Extractions

C. Munding "christine.munding" at NOSPAM.cell.biol.ethz.ch
Wed Oct 25 05:45:28 EST 2000


I am searching for a neat technique to isolate nuclear and cytoplasmatic
proteins. The problem is that the protein I am interested in seems to be
partially membrane bound or insoluble, because i could find it in the
nuclear lysate as well as in the cytoplasmatic and pellet fraction using
the tequenique for isolating nuclaer proteins for band shift assays.
Does anybody have a better idea?
christine. munding at cell.biol.ethz.ch






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