safety: Immunocytochemistry with frozen human tissue

peachii at peachii at
Wed Oct 25 18:03:10 EST 2000

Does anyone out there have safety suggestions for working with unfixed
frozen human
tissues? I want to cut (supposedly normal) human tissue on a cryostat
not typically used for
biohazard materials.

Also, I have heard that brief fixation in cold acetone will immobilize
most biohazard threat
from this tissue, but I have concerns about the effectiveness of this,
and the damage it will
do to morphology.

Any suggestions would be greatly appreciated!

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