safety: immunocytochemistry with frozen human tissues

peachii at peachii at
Wed Oct 25 18:02:12 EST 2000

Does anyone out there have safety suggestions for working with unfixed frozen
human tissues? I want to cut (supposedly normal) human tissue on a cryostat
not typically used for biohazard materials.

Also, I have heard that brief fixation in cold acetone will immobilize most
biohazard threat from this tissue, but I have concerns about the
effectiveness of this, and the damage it will do to morphology.

Any suggestions would be greatly appreciated!

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