HUVECs fixation for NF kappa B

Tomas Corcoran mascor at gofree.indigo.ie
Thu Oct 26 08:47:10 EST 2000


Can anyone suggest a validated method for the permeabilisation of HUVECs
without altering their NFkappa B status in order that I can stain them with
a primary p65 antibody and then a secondary labelled IgG

Many thanks


Tomas Corcoran

mascor at gofree.indigo.ie







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