Mitomycin inactivated embryonic fibroblasts die after 12 hours

Philippe Heymans Philippe.Heymans at
Fri Oct 27 09:32:56 EST 2000

Does anybody know why inactivation with mitomycin 10ug/ml (2h at 37 C) is
not supported by embryonic fibroblasts ? They attach during the 3.4 hours
until approximately 40%, and then die the next morning. Medium is standard
with gentamycin, FCS is 15%, CO2 is 5%.
Thank you !!!

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