Ceramide ELISA

Jens Rainer Hansen jens.rainer at leo.dk
Fri Sep 8 05:37:00 EST 2000


I have coated ELISA plates with Ceramide - 1000 - 100 - 10 and 0 ng/ml (100
mcl/well) using 2 coating buffres (ph 9.6 and 7.2).

Hereafter we added a mouse anti ceramide antibody and a biotinylated rabbit
anti mouse antibody, TMB etc., with no result. All wells gave the same as
the "0" = bagground.

Does anybody have experience with coating procedures using ceramide ?


Jens R.









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