Glycerol shock in transfection

dinalt dinalt at ukonline.co.uk
Sun Sep 24 11:20:12 EST 2000


Hi,

Does anyone know how glycerol shock actually aids DNA uptake by cells or
cell nuclei during cell transfection, or does anyone have a reference
describing the mechanism (not the actual protocol tho'!)? Pubmed appears to
have references that use the technique but none explaining why it works.

Thanks in advance,
David Zeitlin







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