rgd bacterial adhesion assay

Robert Hartley rh at mblab.gla.ac.uk
Fri Sep 29 05:43:33 EST 2000


Hello all.
A colleague wants to perform an adhesion assay for bacterial surface
protein epitopes, specifically an xRGD and SGxG sequences.

I thought of an integrin, say a3b1 located within something like matrigel
or using an Ab to the epitopes. another would be a simple monolayer of
epithellial/endothellial cells which express the integrin. To ovecome the
problem of other bacterial adhesion sites interacting with the eukaryotic
monolayer, one could use a small rgd containing peptide and do a
compettitive inhibition assay.

These are a bit of a guess however.

Does anyone have a bog standard assay for sequence specific baterial adhesion.

Cheers
Bob; Sunny Scotland

-- 
Robert Hartley, Centre for Cell Engineering,Joseph Black Building
University of Glasgow, Glasgow G12 8QQ Tel: ++44 (0)141 330 4756, 
Fax: 0141 330 3730 mailto:rh at mblab.gla.ac.uk
Web : http://www.gla.ac.uk/Inter/CellEngineering






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