R: Cell sonication methods

Michael Witty mw132 at mole.bio.cam.ac.uk
Sat Apr 28 10:38:04 EST 2001


Dear Ken,
        are these vascular endothelial cells of plant origin?  If so
wouldn't grinding with a little fine sand be better and easier.  Possibly
grinding material frozen in liquid nitrogen?  Mike.

On Sat, 28 Apr 2001, marietto wrote:

> Dear Ken, I have the same problem but I was told to sonicate from one to X
> times and from few to twenty seconds each time. After that I ought to see an
> aliquot of my sample under a microscope. If I do not see the cells, it means
> that the rupturing has been successful. Don't forget to take a look at an
> aliquot from non-sonicated cells as a control.
> Hope it can help.
> Please reply at
> squinzanetto at libero.it
> squinz at hotmail.com
>
> Bio-Stress <bio-stress at home.com> wrote in message
> 3ACBD679.DCE76644 at home.com...
> >
> > I'm trying to find some information on rupturing of cells via sonication:
> > how long sonication, how long on ice etc. These are vascular endothelial
> cells.
> > I know it depends on cell type but I need a starting point on where to
> > begin. After sonication what methods should be used to determine the
> "success"
> > of the rupturing?
> >
> > Thank you.
> >
> > Ken
> > --
> > Kenneth A. Hoekstra
> > Faculty of Agricultural Sciences
> > University of British Columbia
> > 248-2357 Main Mall
> > Vancouver, BC, Canada, V6T 1Z4
> > Tel:  (604) 822-1422
> > Fax: (604) 822-4400
> > Email: hoekstra at interchange.ubc.ca
>
>
>




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