immunoelectron microscopy

INSERM U189 U189 at
Mon Apr 30 10:02:40 EST 2001

I am working on a protein, and I try to visualize it with immunoelectron
microscopy. I use a pre-embedding immunogold method. Analyzes were  not
simple, I think, because I use 5nm gold particles, and I see (in TEM)
electron dense particles in the cells, but the size of these seems to be
lightly different. Can someone explain me that ? perhaps because the
distribution of the protein in all the thickness of the section ?
Thanks in advance


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