Bt Toxin Resistance from Loss of a Putative Carbohydrate-Modifying Enzyme

Rcjohnsen rcjohnsen at aol.com
Wed Aug 8 19:09:40 EST 2001


Medicine/Diseases  

Bt Toxin Resistance from Loss of a Putative Carbohydrate-Modifying Enzyme Sci
293:860  8-3-01

Joel S. Griffitts, Johanna L. Whitacre, Daniel E. Stevens, Raffi V. Aroian*  
The development of resistance is the main threat to the long-term use of toxins
from Bacillus thuringiensis (Bt) in transgenic plants. Here we report the
cloning of a Bt toxin resistance gene, Caenorhabditis elegans bre-5, which
encodes a putative -1,3-galactosyltransferase. Lack of bre-5 in the intestine
led to resistance to the Bt toxin Cry5B. Wild-type but not bre-5 mutant animals
were found to uptake toxin into their gut cells, consistent with bre-5 mutants
lacking toxin-binding sites on their apical gut. bre-5 mutants displayed
resistance to Cry14A, a Bt toxin lethal to both nematodes and insects; this
indicates that resistance by loss of carbohydrate modification is relevant to
multiple Bt toxins.  
Section of Cell and Developmental Biology, University of California, San Diego,
La Jolla, CA 92093, USA. 
*   To whom correspondence should be addressed. E-mail: raroian at ucsd.edu 

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