Purifying antibody

Robert Somogyi somogyi at aecom.yu.edu
Tue Aug 14 11:50:55 EST 2001


Pierce sells kits for making columns with your peptide. I used the Sulfo
link kit (cat # 20401/2) which is designed for that cys residue
www.piercenet.com

good luck

Robert

On Tue, 14 Aug 2001 gs207 at yahoo.com wrote:

> Hi
> 
> I am new to the field of Immunology etc, so please excuse me if I ask
> something stupid.  I want to use an antibody in immunoprecipitation
> assays .  All I have been provided with are bleeds from rabbit immunized
> with peptide from the protein of interest.  The peptide has a cysteine
> at amino terminus.  I have been told that I need to purify that antibody
> with that peptide before using it.  I have no idea how to go about it.
> I would really appreciate any help with this.  If you can guide me to a
> published protocol (better if you can provide me with one you have used)
> and/or any kits I can use.  Thanks a lot.
> 
> 
> 




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