Trypan for attached cells...

Tom Fletcher tfletch at pacbell.net
Thu Dec 20 00:35:18 EST 2001


Cells are normally trypsinzed and suspended before using the trypan blue.
This is necessary for them to be counted in a hemacytometer.
"bryan" <bryan at photon.unm.edu> wrote in message
news:3C1A4534.218F70B6 at photon.unm.edu...
>
> i cant find any protocols for using the trypan blue viability assay on
> attached cells....Can this be done???? i cant image why not, but it
> seems to be quite uncommon...does anyone have a protocol for this???
>
> ---





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