GST and DISULFIDE BONDS
pmoreno at servidor.ibv.csic.es
Fri Feb 23 04:54:44 EST 2001
I´m expressing a protein fusion GST. My protein has disulfide bonds,
but i would like to know if my protein has some free cys. For that, I´m
treating it with urea and after with "eosin-iodoacetamide" (it link with
a free SH-). But I don´t know if GST has a free cys. I know GST come
from Schistosoma japonicum, has 4 cys.
Someone knows how many has free?
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