in situ hybe

Rachel rgeddy at po-box.mcgill.ca
Mon Jan 29 21:06:58 EST 2001


I am performing a radioactive in situ hybridization study and have come
to the point of developing my slides. Two tester slides have me quite
confused. Is the NTB-2 emulsion supposed to stay on the slide,
effectively covering the sections, or is it supposed to come off to
reveal the silver grains? All protocols I have seen neglect to mention
this detail, and I have one slide with a emulsion layer which won't come
off and one whose emulsion layer is coming off in pieces.

Thanks for your advice.

Rachel Geddy
McGill University






More information about the Cellbiol mailing list