A Transgenic Model for Listeriosis

Rcjohnsen rcjohnsen at aol.com
Sun Jul 8 23:30:35 EST 2001


Microbiology  

A Transgenic Model for Listeriosis: Role of Internalin in Crossing the
Intestinal Barrier 

Marc Lecuit,1 Sandrine Vandormael-Pournin,2 Jean Lefort,3 Michel Huerre,4
Pierre Gounon,5 Catherine Dupuy,1 Charles Babinet,2 Pascale Cossart1*  
Listeria monocytogenes is responsible for severe food-borne infections, but the
mechanisms by which bacteria cross the intestinal barrier are unknown. Listeria
monocytogenes expresses a surface protein, internalin, that interacts with a
host receptor, E-cadherin, to promote entry into human epithelial cells. Murine
E-cadherin, in contrast to guinea pig E-cadherin, does not interact with
internalin, excluding the mouse as a model for addressing internalin function
in vivo. In guinea pigs and transgenic mice expressing human E-cadherin,
internalin was found to mediate invasion of enterocytes and crossing of the
intestinal barrier. These results illustrate how relevant animal models for
human infections can be generated.  
1 Unité des Interactions Bactéries-Cellules, 
2 Unité de Biologie du Développement, Unité de Recherche Associée CNRS 1960, 
3 Unité de Pharmacologie Cellulaire, 
4 Unité d'Histopathologie, 
5 Station Centrale de Microscopie Electronique, Institut Pasteur, 75724 Paris
cedex 15, France. 
*   To whom correspondence should be addressed. E-mail: pcossart at pasteur.fr 



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Volume 292, Number 5522, Issue of 1 Jun 2001, pp. 1722-1725. 
Copyright © 2001 by The American Association for the Advancement of Science.  

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