Complete Genome Sequence of a Virulent Isolate of Streptococcus pneumoniae

Rcjohnsen rcjohnsen at
Sun Jul 22 20:34:54 EST 2001


Complete Genome Sequence of a Virulent Isolate of Streptococcus pneumoniae 

Hervé Tettelin,1 Karen E. Nelson,1 Ian T. Paulsen,12 Jonathan A. Eisen,12
Timothy D. Read,1 Scott Peterson,13 John Heidelberg,1 Robert T. DeBoy,1 Daniel
H. Haft,1 Robert J. Dodson,1 A. Scott Durkin,1 Michelle Gwinn,1 James F.
Kolonay,1 William C. Nelson,1 Jeremy D. Peterson,1 Lowell A. Umayam,1 Owen
White,1 Steven L. Salzberg,14 Matthew R. Lewis,1 Diana Radune,1 Erik
Holtzapple,1 Hoda Khouri,1 Alex M. Wolf,1 Terry R. Utterback,1 Cheryl L.
Hansen,1 Lisa A. McDonald,1 Tamara V. Feldblyum,1 Samuel Angiuoli,1 Tanja
Dickinson,1 Erin K. Hickey,1 Ingeborg E. Holt,1 Brendan J. Loftus,1 Fan Yang,1
Hamilton O. Smith,1* J. Craig Venter,1* Brian A. Dougherty,5 Donald A.
Morrison,6 Susan K. Hollingshead,7 Claire M. Fraser13  
The 2,160,837-base pair genome sequence of an isolate of Streptococcus
pneumoniae, a Gram-positive pathogen that causes pneumonia, bacteremia,
meningitis, and otitis media, contains 2236 predicted coding regions; of these,
1440 (64%) were assigned a biological role. Approximately 5% of the genome is
composed of insertion sequences that may contribute to genome rearrangements
through uptake of foreign DNA. Extracellular enzyme systems for the metabolism
of polysaccharides and hexosamines provide a substantial source of carbon and
nitrogen for S. pneumoniae and also damage host tissues and facilitate
colonization. A motif identified within the signal peptide of proteins is
potentially involved in targeting these proteins to the cell surface of
low-guanine/cytosine (GC) Gram-positive species. Several surface-exposed
proteins that may serve as potential vaccine candidates were identified.
Comparative genome hybridization with DNA arrays revealed strain differences in
S. pneumoniae that could contribute to differences in virulence and
1 The Institute for Genomic Research (TIGR), 9712 Medical Center Drive,
Rockville, MD 20850, USA. 
2 Johns Hopkins University, Charles and 34th Streets, Baltimore, MD 21218, USA.

3 George Washington University Medical Center, 2300 Eye Street, NW, Washington,
DC 20037, USA. 
4 Johns Hopkins University, 3400 North Charles Street, Baltimore, MD
21218, USA. 
5 Bristol-Myers Squibb PRI, 5 Research Parkway, Wallingford, CT 06492, USA. 
6 University of Illinois at Chicago, 900 South Ashland Avenue, Chicago, IL
60607, USA. 
7 University of Alabama at Birmingham, 845 19th Street South, Birmingham, AL
35294, USA. 
*   Present address: Celera Genomics, 45 West Gude Drive, Rockville, MD
20850, USA. 

   To whom correspondence should be addressed. E-mail: cmfraser at 


Related articles in Science:

S. pneumoniae Genome Falls to Sequencers.

Dan Ferber 
Science 2001 293: 410. (in News of the Week) [Summary] [Full Text]   

Volume 293, Number 5529, Issue of 20 Jul 2001, pp. 498-506. 
Copyright © 2001 by The American Association for the Advancement of Science.  

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