RNA isolated from the priamry neurons

[Jinhongwei]

 i am newer comer in this field, i wanna study channels genes from the
primary cultured hippocampal neuron using RT-PCR.
if i use Trizol to isolat RNA for neurons,how to deal the cells before
adding Trizol using scraping cutter or trypsin disgestion


<http://www.biowww.net/forum/read.php?f=1&i=1024&t=1024>