6XHis Antibody

[Nathan Alder]

     I am currently working with protein bearing a 6X-His tag on the
C-terminal end overexpressed from a Novagen pET system.  I have been
working on a protocol for protein detection using a hexa-His probe
antibody with partial success.  Has anyone used a 6XHis detection system
which works with a high sensitivity? Any information/suggestions on this
matter would be greatly appreciated.

Nathan Alder
UC Davis
Life Sciences Addition