6XHis Antibody

Michael Witty mw132 at mole.bio.cam.ac.uk
Sun Mar 11 09:08:25 EST 2001


Nathan,
      can you see your protein in a crude extract on SDS-PAGE? or can you
use Ni-NTA to purify/concentrate your protein?

Mike.

On Fri, 9 Mar 2001, Nathan Alder wrote:

> 
>      I am currently working with protein bearing a 6X-His tag on the
> C-terminal end overexpressed from a Novagen pET system.  I have been
> working on a protocol for protein detection using a hexa-His probe
> antibody with partial success.  Has anyone used a 6XHis detection system
> which works with a high sensitivity? Any information/suggestions on this
> matter would be greatly appreciated.
> 
> Nathan Alder
> UC Davis
> Life Sciences Addition
> 
> 
> 
> 






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