coating coverslips with fibronectin

Sophie Le Page slepage at umich.edu
Wed Mar 14 16:11:05 EST 2001


Hi everybody,

I am trying to find a reliable method for coating coverslips with
fibronectin. So far (from trawling the web) I have come up with:

5ug/cm fibronectin put onto the coverslip for 1hr @ 37C

wash 2x with PBS and use coverslips immediately.

Does this sound reasonable?? Any further suggestions?

Thanks,

Sophie




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