localization of receptor

Tina anfortin at webnet.qc.ca
Fri Mar 16 20:19:48 EST 2001


Is there a method to distinguish between intracellular receptors (internal
stores, pool) and receptors synthetized de novo ? I stimulated PMNs with a
cytokine and I want to know if the enhanced expression of a receptor is
related to de novo synthesis or expression of an internal stock. Is it
possible by Western blot combined with an other technique of subcellular
fractionnation?

Thanks a lot.





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