localization of receptor

Bryan Heit bjheit at ucalgary.ca
Thu Mar 22 12:26:00 EST 2001

I missed the original  post, so this may have been said already...

Have you considered using labeled antibodies to the receptor?  You can use
metal-labeled Ab's for electron microscopy, or you can use fluorescent
labeled Ab's for fluorescent microscopy.  This is a common method to
detect the location of a receptor.

Another possibility is to create a fusion protein (say receptor-GFP).  You
can then track the location of the receptor using fluorescent microscopy.
However, you should back these results up with another experiment to show
that GFP did not alter the localization patterns.

Bryan Heit

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