tme at biomedicaldesign.com
Mon Apr 15 08:15:52 EST 2002
I am trying to adapt the MTT assay for use in 6 well plates and a
spectrometer (not a microplate reader). I have been running into
several problems and have a few questions:
1: Is the formazan released into the medium after solubilization or is
it maintained in the cells?
2: I found an old lab protocol which uses Hanks BSS for the incubation
period but have been unable to find any precedence for this?
3: I am also unable to find any references to this assay being
performed in anything other than 96 well plates. Is there a reason for
this? Any suggestions as to where I might find references for the MTT
assay in other than 96 well plates?
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