Growing HeLa cells

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Mon Jul 15 19:51:02 EST 2002


On Mon, 15 Jul 2002 14:35:24 -0600, Jes Kuruvilla
<jkuruvil at lamar.colostate.edu> wrote:

>Hi all,
>Does anybody know how to get He La cells (spinner adapted)
>to grow successfully? I need to make some nuclear extract. I
>have tried ( Ca- deficient) Jokliks media with 10% fetal
>Calf serum and have been keeping the density at 2X 10^5.
>They still seem to be just sitting around in the flask and
>not doubling. Most of the cells are found in clumps. Is this
>usual for cells taken from a trypsinized 10 cm plate??
>

Which "strain" of HeLa's are you using? When I was in grad. school, it
was "lab dogma" that HeLa S3 could grow in spinners (and they did)
whereas our other strain did poorly in spinners.

Nick

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu



More information about the Cellbiol mailing list